Analysis of Internet Diffusion in Italy by means of domain names among firms, associations and individuals

This paper analyzes Internet diffusion among a number of organizations and individuals, based on daily observation of second-level domain names registrations under the ".it" ccTLD. In particular, we analyzed domain names registered by associations, firms and individuals. The penetration rate, calculated according to the population, was computed for various widely separated geographic levels (provinces and regions). Results show that a "digital divide" exists in terms of geographical distribution (i.e., in macro-areas - Northern, Central, and Southern Italy - at a regional level and at a provincial level) and that such geographical distribution is more concentrated compared to Italian population and total income, suggesting a diffusive effect

Studio della diffusione di Internet attraverso l\u27uso dei nomi a dominio sotto il ccTLD ".it", nelle aree del territorio regionale toscano che non sono raggiunte dal servizio di connettivit? a banda larga.

In italian onlyIl presente studio ha l\u27obiettivo di verificare l\u27uso di Internet al 31-12-2007 nelle aree (comuni) oggetto d\u27intervento da parte della regione Toscana nella fornitura dei servizi di banda larga. Tali aree non sono oggi raggiunte dal servizio di connettivit? a causa del fallimento del mercato. L\u27obiettivo ? quindi di verificare lo stato dell\u27uso di Internet nei comuni toscani in modo da riuscire a valutare se, in futuro, le politiche adottate dalla Regione potranno avere un impatto positivo nella diffusione della rete in tali aree. Per determinare lo stato attuale della diffusione di Internet nelle aree oggetto di intervento si ? utilizzato come indicatore il numero dei domini registrati sotto il ccTLD (country code Top Level Domain) ".it", gestito dall\u27Istituto di Informatica e Telematica del CNR

Studio sulla diffusione di Internet nella regione Toscana

Available in ItalianPer analizzare la diffusione di Internet a livello toscano abbiamo utilizzato l\u27indicatore endogeno dei nomi di dominio contenuti nei database del Registro del ccTLD (acronimo di country code Top Level Domain) .it, gestito dallo IIT-CNR. L\u27analisi mostra la presenza del Digital Divide sia a livello regionale che provinciale

A Framework to Evaluate Information Quality in Public Administration Website

This paper presents a framework aimed at assessing the capacity of Public Administration bodies (PA) to offer a good quality of information and service on their web portals. Our framework is based on the extraction of “.it” domain names registered by Italian public institutions and the subsequent analysis of their relative websites. The analysis foresees an automatic gathering of the web pages of PA portals by means of web crawling and an assessment of the quality of their online information services. This assessment is carried out by verifying their compliance with current legislation on the basis of the criteria established in government guidelines[1]. This approach provides an ongoing monitoring process of the PA websites that can contribute to the improvement of their overall quality. Moreover, our approach can also hopefully be of benefit to local governments in other countries. Available at: https://aisel.aisnet.org/pajais/vol5/iss3/3

Annuario Registro dati 2005

No abstract availabl

Production of a Bacillus anthracis Secretome with Suitable Characteristics as Antigen in a Complement Fixation Test

In this study, we cultured the Bacillus anthracis vaccine strain Sterne 34F2 in a medium containing EDTA, and we assessed the best conditions to inhibit the activity of zinc-dependent metalloproteases to obtain a secretome containing a high concentration of non-degraded PA (PA83), as evaluated by the SDS-PAGE analysis. Then, we used this secretome as the antigen in a Complement Fixation Test (CFT) to monitor the production of antibodies against PA83 in the sera of rabbits vaccinated with Sterne 34F2 and then infected with a B. anthracis virulent strain to evaluate the potency of the vaccine. The PAS-based CFT results were compared with those obtained by using a commercial ELISA kit. The two serological tests gave similar results in terms of specificity and sensitivity, as the kinetics of the antibodies production was very similar. The Sterne 34F2 vaccine induced an antibody response to PA83, whose titer was not inferior to 1:8 in PAS-based CFT and 42 kU/mL in PA83-based ELISA, respectively, in all vaccinated rabbits. Our opinion is that the PAS-based CFT can be successfully employed in humans and in animals for epidemiological retrospective studies or post-vaccination monitoring. We also suggest the use of our method to test the efficacy of veterinary anthrax vaccines

Discrimination of Bacillus cereus Group Members by MALDI-TOF Mass Spectrometry

Matrix-Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry (MALDI-TOF MS) technology is currently increasingly used in diagnostic laboratories as a cost effective, rapid and reliable routine technique for the identification and typing of microorganisms. In this study, we used MALDI-TOF MS to analyze a collection of 160 strains belonging to the Bacillus cereus group (57 B. anthracis, 49 B. cereus, 1 B. mycoides, 18 B. wiedmannii, 27 B. thuringiensis, 7 B. toyonensis and 1 B. weihenstephanensis) and to detect specific biomarkers which would allow an unequivocal identification. The Main Spectra Profiles (MSPs) were added to an in-house reference library, expanding the current commercial library which does not include B. toyonensis and B. wiedmannii mass spectra. The obtained mass spectra were statistically compared by Principal Component Analysis (PCA) that revealed seven different clusters. Moreover, for the identification purpose, were generated dedicate algorithms for a rapid and automatic detection of characteristic ion peaks after the mass spectra acquisition. The presence of specific biomarkers can be used to differentiate strains within the B. cereus group and to make a reliable identification of Bacillus anthracis, etiologic agent of anthrax, which is the most pathogenic and feared bacterium of the group. This could offer a critical time advantage for the diagnosis and for the clinical management of human anthrax even in case of bioterror attacks

Sars-CoV-2 isolation from a 10-day-old newborn in Italy: A case report

This report describes the evolution of COVID-19 in a 10 day-old-baby. The mother developed the disease immediately after childbirth and therefore a vertical transmission can be excluded. The isolation of the virus in cell culture with a cytopathic effect already visible after 48 h, indicates that the viral load of the newborn was quite high, but not serious course of the disease was observed. This paper wants to highlight the possible role of newborns and children in the spread of the disease

Anthrax phylogenetic structure in Northern Italy

<p>Abstract</p> <p>Background</p> <p>Anthrax has almost disappeared from mainland Europe, except for the Mediterranean region where cases are still reported. In Central and South Italy, anthrax is enzootic, but in the North there are currently no high risk areas, with only sporadic cases having been registered in the last few decades. Regional genetic and molecular characterizations of anthrax in these regions are still lacking. To investigate the potential molecular diversity of <it>Bacillus anthracis </it>in Northern Italy, canonical Single nucleotide polymorphism (canSNP) and Multilocus variable number tandem repeat analysis (MLVA) genotyping was performed against all isolates from animal outbreaks registered in the last twenty years in the region.</p> <p>Findings</p> <p>Six <it>B. anthracis </it>strains were analyzed. The canSNP analysis indicates the presence of three sublineages/subgroups each of which belong to one of the 12 worldwide CanSNP genotypes: B.Br.CNEVA (3 isolates), A.Br.005/006 (1 isolates) and A.008/009 (2 isolate). The latter is the dominant canSNP genotype in Italy. The 15-loci MLVA analysis revealed five different genotypes among the isolates.</p> <p>Conclusions</p> <p>The major B branch and the A.Br.005/006 were recovered in the Northeast region. The genetic structure of anthrax discovered in this area differs from the rest of the country, suggesting the presence of a separate and independent <it>B. anthracis </it>molecular evolution niche. Although the isolates analyzed in this study are limited in quantity and representation, these results indicate that <it>B. anthracis </it>genetic diversity changes around the Alps.</p